Journal: PLOS One
Article Title: Butyrate ameliorates DSS-induced ulcerative colitis in mice by facilitating autophagy in intestinal epithelial cells and modulating the gut microbiota through blocking the PI3K-AKT-mTOR pathway
doi: 10.1371/journal.pone.0337214
Figure Lengend Snippet: (A) (B) Western blot of autophagy-related proteins. (C) Western blot of PI3K/AKT/mTOR pathway proteins. (D–J) Quantitative analysis of (D) Beclin-1, (E) Atg5, (F) LC3II/I, (G) P62, (H) p-PI3K/PI3K, (I) p-AKT/AKT, and (J) p-mTOR/mTOR protein levels. Data are mean ± SEM; #P < 0.05, ##P < 0.01 vs Control; *P < 0.05, **P < 0.01 vs DSS group.
Article Snippet: PI3K, P62, and Beclin-1 antibodies were obtained from ABclonal (A19742, A19700, and A21191, Wuhan, China), and phospho-PI3K (p-PI3K) from Bioss (bs-5570R, Beijing, China).
Techniques: Western Blot, Control