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rabbit anti phospho pi3k  (Bioss)


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    Structured Review

    Bioss rabbit anti phospho pi3k
    Rabbit Anti Phospho Pi3k, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+phospho+pi3k/PIK3R2(Tyr467)+Polyclonal+Antibody/pm42096998-129-10-14
    Average 94 stars, based on 3 article reviews
    rabbit anti phospho pi3k - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: TEAD4-mediated upregulation of LPAR3 augments hepatic stellate cell activation in portal hypertension.
    Article Snippet: .. After blocking, the sections were probed with the Myosin IIA antibody (1:500, ab238131, Abcam) at 22–25°C for 2 h, followed by incubation with IgG (Alexa Fluor 488) (1:1000, ab150077, Abcam) at 22–25°C in darkness for 1 h. Additionally, fluorescent primary antibodies were added as needed and incubated in the dark for 1 h. These antibodies included AbBy Fluor 488-conjugated anti-LPAR3 (1:200, bs-2882R-BF488, Bioss Antibodies, Woburn, MA, USA), anti-phospho-p38 MAPK (1:200, bs-23251R-BF488, Bioss), anti-phospho-PI3K (1:200, bs-5571R-BF488, Bioss), antiphospho-AKT (1:200, bs-12456R-BF488, Bioss), anti-TEAD4 (1:200, bs-9603R-BF488, Bioss), and Alexa Fluor 647-conjugated anti-α-SMA (1:200, ITT5797-647, G-Biosciences, St Louis, MO, USA). ..

    Article Title: TEAD4-mediated upregulation of LPAR3 augments hepatic stellate cell activation in portal hypertension
    Article Snippet: .. After blocking, the sections were probed with the Myosin IIA antibody (1:500, ab238131, Abcam) at 22–25°C for 2 h, followed by incubation with IgG (Alexa Fluor 488) (1:1000, ab150077, Abcam) at 22–25°C in darkness for 1 h. Additionally, fluorescent primary antibodies were added as needed and incubated in the dark for 1 h. These antibodies included AbBy Fluor 488-conjugated anti-LPAR3 (1:200, bs-2882R-BF488, Bioss Antibodies, Woburn, MA, USA), anti-phospho-p38 MAPK (1:200, bs-23251R-BF488, Bioss), anti-phospho-PI3K (1:200, bs-5571R-BF488, Bioss), anti-phospho-AKT (1:200, bs-12456R-BF488, Bioss), anti-TEAD4 (1:200, bs-9603R-BF488, Bioss), and Alexa Fluor 647-conjugated anti-α-SMA (1:200, ITT5797-647, G-Biosciences, St Louis, MO, USA). ..

    Article Title: Acupuncture Activates PI3K/Akt Pathway to Promote Spinal Microglial M2 Polarization and Alleviate Spastic Hemiplegia Following Ischemic Stroke
    Article Snippet: .. After blocking with 5% BSA, membranes were incubated overnight at 4°C with the following primary antibodies:anti-CD32 (1:1000, AF4648, Affinity Biosciences), anti-CD206 (1:1000, DF4149, HUABIO), anti-vGAT (1:1000, ab42939, Abcam), anti-vGluT1 (1:2000, ab272913, Abcam), anti-PI3K (1:1000, bs10657R, Bioss), anti-phospho-PI3K (1:2000, bs-6417R, Bioss), anti-Akt (1:2000, bsm-52010R, Bioss), anti-phospho-Akt (1:2000, bsm-52130R, Bioss), and anti-GAPDH (1:5000, bsm-33033M, Bioss). .. After incubating with secondary antibodies, images were visualized using an ECL kit (#34096, Thermo Fisher Scientific) and a chemiluminescence imaging system (Liuyi Biotechnology).

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder
    Article Snippet: .. Membranes were briefly blocked with protein-free blocking buffer (5 min) and incubated with the following primary antibodies at 4 °C overnight: anti-AKT (1:1000, rabbit, GB15689-50, Servicebio), anti-phospho-AKT (1:500, rabbit, WLP001a, WANLEIBIO), anti-PI3K (1:500, rabbit, GB11525-50, Servicebio), anti-phospho-PI3K (1:500, rabbit, bs-5582R, Bioss), anti-mTOR (1:500, rabbit, GB11405-50, Servicebio), and anti-phospho-mTOR (1:500, rabbit, GB114489 , Servicebio). ..

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder.
    Article Snippet: .. Membranes were briefly blocked with protein-free blocking buffer (5 min) and incubated with the following primary antibodies at 4 °C overnight: anti-AKT (1:1000, rabbit, GB15689-50, Servicebio), anti-phospho-AKT (1:500, rabbit, WLP001a, WANLEIBIO), anti-PI3K (1:500, rabbit, GB11525-50, Servicebio), anti-phospho-PI3K (1:500, rabbit, bs-5582R, Bioss), anti-mTOR (1:500, rabbit, GB11405-50, Servicebio), and anti-phospho-mTOR (1:500, rabbit, GB114489, Servicebio). ..

    Article Title: Acupuncture Activates PI3K/Akt Pathway to Promote Spinal Microglial M2 Polarization and Alleviate Spastic Hemiplegia Following Ischemic Stroke
    Article Snippet: .. After blocking with 5% BSA, membranes were incubated overnight at 4°C with the following primary antibodies:anti-CD32 (1:1000, AF4648, Affinity Biosciences), anti-CD206 (1:1000, DF4149, HUABIO), anti-vGAT (1:1000, ab42939, Abcam), anti-vGluT1 (1:2000, ab272913, Abcam), anti-PI3K (1:1000, bs-10657R, Bioss), anti-phospho-PI3K (1:2000, bs-6417R, Bioss), anti-Akt (1:2000, bsm-52010R, Bioss), anti-phospho-Akt (1:2000, bsm-52130R, Bioss), and anti-GAPDH (1:5000, bsm-33033M, Bioss). .. After incubating with secondary antibodies, images were visualized using an ECL kit (#34096, Thermo Fisher Scientific) and a chemiluminescence imaging system (Liuyi Biotechnology).

    Incubation:

    Article Title: TEAD4-mediated upregulation of LPAR3 augments hepatic stellate cell activation in portal hypertension.
    Article Snippet: .. After blocking, the sections were probed with the Myosin IIA antibody (1:500, ab238131, Abcam) at 22–25°C for 2 h, followed by incubation with IgG (Alexa Fluor 488) (1:1000, ab150077, Abcam) at 22–25°C in darkness for 1 h. Additionally, fluorescent primary antibodies were added as needed and incubated in the dark for 1 h. These antibodies included AbBy Fluor 488-conjugated anti-LPAR3 (1:200, bs-2882R-BF488, Bioss Antibodies, Woburn, MA, USA), anti-phospho-p38 MAPK (1:200, bs-23251R-BF488, Bioss), anti-phospho-PI3K (1:200, bs-5571R-BF488, Bioss), antiphospho-AKT (1:200, bs-12456R-BF488, Bioss), anti-TEAD4 (1:200, bs-9603R-BF488, Bioss), and Alexa Fluor 647-conjugated anti-α-SMA (1:200, ITT5797-647, G-Biosciences, St Louis, MO, USA). ..

    Article Title: TEAD4-mediated upregulation of LPAR3 augments hepatic stellate cell activation in portal hypertension
    Article Snippet: .. After blocking, the sections were probed with the Myosin IIA antibody (1:500, ab238131, Abcam) at 22–25°C for 2 h, followed by incubation with IgG (Alexa Fluor 488) (1:1000, ab150077, Abcam) at 22–25°C in darkness for 1 h. Additionally, fluorescent primary antibodies were added as needed and incubated in the dark for 1 h. These antibodies included AbBy Fluor 488-conjugated anti-LPAR3 (1:200, bs-2882R-BF488, Bioss Antibodies, Woburn, MA, USA), anti-phospho-p38 MAPK (1:200, bs-23251R-BF488, Bioss), anti-phospho-PI3K (1:200, bs-5571R-BF488, Bioss), anti-phospho-AKT (1:200, bs-12456R-BF488, Bioss), anti-TEAD4 (1:200, bs-9603R-BF488, Bioss), and Alexa Fluor 647-conjugated anti-α-SMA (1:200, ITT5797-647, G-Biosciences, St Louis, MO, USA). ..

    Article Title: Acupuncture Activates PI3K/Akt Pathway to Promote Spinal Microglial M2 Polarization and Alleviate Spastic Hemiplegia Following Ischemic Stroke
    Article Snippet: .. After blocking with 5% BSA, membranes were incubated overnight at 4°C with the following primary antibodies:anti-CD32 (1:1000, AF4648, Affinity Biosciences), anti-CD206 (1:1000, DF4149, HUABIO), anti-vGAT (1:1000, ab42939, Abcam), anti-vGluT1 (1:2000, ab272913, Abcam), anti-PI3K (1:1000, bs10657R, Bioss), anti-phospho-PI3K (1:2000, bs-6417R, Bioss), anti-Akt (1:2000, bsm-52010R, Bioss), anti-phospho-Akt (1:2000, bsm-52130R, Bioss), and anti-GAPDH (1:5000, bsm-33033M, Bioss). .. After incubating with secondary antibodies, images were visualized using an ECL kit (#34096, Thermo Fisher Scientific) and a chemiluminescence imaging system (Liuyi Biotechnology).

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder
    Article Snippet: .. Membranes were briefly blocked with protein-free blocking buffer (5 min) and incubated with the following primary antibodies at 4 °C overnight: anti-AKT (1:1000, rabbit, GB15689-50, Servicebio), anti-phospho-AKT (1:500, rabbit, WLP001a, WANLEIBIO), anti-PI3K (1:500, rabbit, GB11525-50, Servicebio), anti-phospho-PI3K (1:500, rabbit, bs-5582R, Bioss), anti-mTOR (1:500, rabbit, GB11405-50, Servicebio), and anti-phospho-mTOR (1:500, rabbit, GB114489 , Servicebio). ..

    Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder.
    Article Snippet: .. Membranes were briefly blocked with protein-free blocking buffer (5 min) and incubated with the following primary antibodies at 4 °C overnight: anti-AKT (1:1000, rabbit, GB15689-50, Servicebio), anti-phospho-AKT (1:500, rabbit, WLP001a, WANLEIBIO), anti-PI3K (1:500, rabbit, GB11525-50, Servicebio), anti-phospho-PI3K (1:500, rabbit, bs-5582R, Bioss), anti-mTOR (1:500, rabbit, GB11405-50, Servicebio), and anti-phospho-mTOR (1:500, rabbit, GB114489, Servicebio). ..

    Article Title: Liraglutide modulates ALCAT1-Mediated cardiolipin remodeling to improve cardiac function in obese mice.
    Article Snippet: Obesity, a significant risk factor for cardiovascular diseases, induces cardiolipin (CL) remodeling.. Acyl-CoA: lysocardiolipin acyltransferase-1 (ALCAT1), a key enzyme in CL metabolism, drives mitochondrial impairment and cardiac dysfunction in obesity.. Although glucagon-like peptide-1 receptor agonists (GLP-1RAs) exhibit cardioprotective properties, their effects on ALCAT1-mediated CL remodeling in obesity-induced myocardial injury remain unclear.

    Article Title: Acupuncture Activates PI3K/Akt Pathway to Promote Spinal Microglial M2 Polarization and Alleviate Spastic Hemiplegia Following Ischemic Stroke
    Article Snippet: .. After blocking with 5% BSA, membranes were incubated overnight at 4°C with the following primary antibodies:anti-CD32 (1:1000, AF4648, Affinity Biosciences), anti-CD206 (1:1000, DF4149, HUABIO), anti-vGAT (1:1000, ab42939, Abcam), anti-vGluT1 (1:2000, ab272913, Abcam), anti-PI3K (1:1000, bs-10657R, Bioss), anti-phospho-PI3K (1:2000, bs-6417R, Bioss), anti-Akt (1:2000, bsm-52010R, Bioss), anti-phospho-Akt (1:2000, bsm-52130R, Bioss), and anti-GAPDH (1:5000, bsm-33033M, Bioss). .. After incubating with secondary antibodies, images were visualized using an ECL kit (#34096, Thermo Fisher Scientific) and a chemiluminescence imaging system (Liuyi Biotechnology).

    Membrane:

    Article Title: Liraglutide modulates ALCAT1-Mediated cardiolipin remodeling to improve cardiac function in obese mice.
    Article Snippet: Obesity, a significant risk factor for cardiovascular diseases, induces cardiolipin (CL) remodeling.. Acyl-CoA: lysocardiolipin acyltransferase-1 (ALCAT1), a key enzyme in CL metabolism, drives mitochondrial impairment and cardiac dysfunction in obesity.. Although glucagon-like peptide-1 receptor agonists (GLP-1RAs) exhibit cardioprotective properties, their effects on ALCAT1-mediated CL remodeling in obesity-induced myocardial injury remain unclear.



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    Image Search Results


    Tryptophanol treatment alleviated POF symptoms by inhibiting PI3K/AKT and MAPK pathways. A Experimental scheme. B Estrus status of mice ( n = 8). C Body weights of mice ( n = 8). D Ovary weights of mice ( n = 8). E Representative Ovary from the Control, the POF and the NCG groups mice with H&E and IHC staining. H&E scale bar = 200 μm; IHC scale bar = 100 μm. F Quantification of primordial, primary, secondary, antral follicles, corpora lutea and corpora atretica per ovary. n = 3 ovaries per each condition. Levels of FSH ( G ), LH ( H ), E 2 ( I ) in mouse serum ( n = 6). Levels of ROS ( J ), MDA ( K ), SOD ( L ), GSH-Px ( M ) in mouse ovaries ( n = 3–4). N Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). O Litter sizes of mice ( n = 3). Data presented as the mean ± SD, statistical significance was determined using one-way or two-way ANOVA. * p < 0.05, ** p < 0.01. *** p < 0.001. Different lowercase letters indicate significant differences ( p < 0.05)

    Journal: Journal of Ovarian Research

    Article Title: Gut microbiota-derived Tryptophanol driven by N-Carbamylglutamate alleviates premature ovarian failure through inhibiting oxidative stress

    doi: 10.1186/s13048-026-02152-4

    Figure Lengend Snippet: Tryptophanol treatment alleviated POF symptoms by inhibiting PI3K/AKT and MAPK pathways. A Experimental scheme. B Estrus status of mice ( n = 8). C Body weights of mice ( n = 8). D Ovary weights of mice ( n = 8). E Representative Ovary from the Control, the POF and the NCG groups mice with H&E and IHC staining. H&E scale bar = 200 μm; IHC scale bar = 100 μm. F Quantification of primordial, primary, secondary, antral follicles, corpora lutea and corpora atretica per ovary. n = 3 ovaries per each condition. Levels of FSH ( G ), LH ( H ), E 2 ( I ) in mouse serum ( n = 6). Levels of ROS ( J ), MDA ( K ), SOD ( L ), GSH-Px ( M ) in mouse ovaries ( n = 3–4). N Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). O Litter sizes of mice ( n = 3). Data presented as the mean ± SD, statistical significance was determined using one-way or two-way ANOVA. * p < 0.05, ** p < 0.01. *** p < 0.001. Different lowercase letters indicate significant differences ( p < 0.05)

    Article Snippet: The membrane was blocked with 5% bovine serum albumin (BSA; Sigma) for 1 h at room temperature, followed by overnight incubation at 4 °C with the primary antibodies: rabbit Cleaved caspase 3 (MCE, HY- P80623 , 1:800), rabbit BAX (Bioworld, BS61098, 1:800), rabbit BCL2 (Bioworld, BS1511, 1:800), rabbit ERK1/2 (Bioworld, BS90472, 1:1,000), rabbit p-ERK1/2 (Bioworld, BS4621P, 1:1,000), rabbit p38 (Bioworld, BS4635, 1:1,000), rabbit p-p38 (Bioworld, BS4766, 1:1,000), rabbit PI3K (MCE, HY- P80867 , 1:1,000); rabbit p-PI3K (MCE, HY- P80846 , 1:1,000), rabbit AKT (Bioworld, MB3288 1:1,000); rabbit p-AKT (Bioworld, BS4007 1:1,000); rabbit IL-6 (Abclonal, A0286, 1:800), rabbit IL-1β (MCE, HY- P80720 , 1:1,000), rabbit Occludin (Abclonal, A2601, 1:1,000); rabbit ZO-1 (Bioworld, BS71522, 1:1,000); rabbit GPX4 (MCE, HY- P80450 , 1:1,000); mouse GAPDH (Bioworld, MB001, 1:1,000); rabbit SOD2 (Bioworld, BS6734, 1:1,000).

    Techniques: Control, Immunohistochemistry, Western Blot

    Tryptophanol treatment alleviated POF by inhibiting PI3K/AKT and MAPK pathways in vitro . A Experimental scheme. B Different concentrations of Cy on GCs viability was assessed by CCK8 assay. C and E Different concentrations of Trp on GCs viability was assessed by CCK8 assay. D The effect of Trp on GCs cell viability following Cy treatment. The control and treatment groups were stained with DCFH-DA, the cells were washed and examined by fluorescence microscopy. Scale bar = 50 μm. F The fluorescence intensity of ROS in mouse GCs. G The level of MDA in mouse GCs. H Flow cytometry apoptosis analysis graph and cell death rate in different groups. The level of SOD ( I ), GSH-Px ( J ), and E 2 ( K ) in mouse GCs. ( L ) Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). All values are presented as the mean ± SD ( n = 3). Different lowercase letters indicate significant differences compared to the control group ( p < 0.05)

    Journal: Journal of Ovarian Research

    Article Title: Gut microbiota-derived Tryptophanol driven by N-Carbamylglutamate alleviates premature ovarian failure through inhibiting oxidative stress

    doi: 10.1186/s13048-026-02152-4

    Figure Lengend Snippet: Tryptophanol treatment alleviated POF by inhibiting PI3K/AKT and MAPK pathways in vitro . A Experimental scheme. B Different concentrations of Cy on GCs viability was assessed by CCK8 assay. C and E Different concentrations of Trp on GCs viability was assessed by CCK8 assay. D The effect of Trp on GCs cell viability following Cy treatment. The control and treatment groups were stained with DCFH-DA, the cells were washed and examined by fluorescence microscopy. Scale bar = 50 μm. F The fluorescence intensity of ROS in mouse GCs. G The level of MDA in mouse GCs. H Flow cytometry apoptosis analysis graph and cell death rate in different groups. The level of SOD ( I ), GSH-Px ( J ), and E 2 ( K ) in mouse GCs. ( L ) Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). All values are presented as the mean ± SD ( n = 3). Different lowercase letters indicate significant differences compared to the control group ( p < 0.05)

    Article Snippet: The membrane was blocked with 5% bovine serum albumin (BSA; Sigma) for 1 h at room temperature, followed by overnight incubation at 4 °C with the primary antibodies: rabbit Cleaved caspase 3 (MCE, HY- P80623 , 1:800), rabbit BAX (Bioworld, BS61098, 1:800), rabbit BCL2 (Bioworld, BS1511, 1:800), rabbit ERK1/2 (Bioworld, BS90472, 1:1,000), rabbit p-ERK1/2 (Bioworld, BS4621P, 1:1,000), rabbit p38 (Bioworld, BS4635, 1:1,000), rabbit p-p38 (Bioworld, BS4766, 1:1,000), rabbit PI3K (MCE, HY- P80867 , 1:1,000); rabbit p-PI3K (MCE, HY- P80846 , 1:1,000), rabbit AKT (Bioworld, MB3288 1:1,000); rabbit p-AKT (Bioworld, BS4007 1:1,000); rabbit IL-6 (Abclonal, A0286, 1:800), rabbit IL-1β (MCE, HY- P80720 , 1:1,000), rabbit Occludin (Abclonal, A2601, 1:1,000); rabbit ZO-1 (Bioworld, BS71522, 1:1,000); rabbit GPX4 (MCE, HY- P80450 , 1:1,000); mouse GAPDH (Bioworld, MB001, 1:1,000); rabbit SOD2 (Bioworld, BS6734, 1:1,000).

    Techniques: In Vitro, CCK-8 Assay, Control, Staining, Fluorescence, Microscopy, Flow Cytometry, Western Blot